INVESTIGATION OF CHEMICAL CONSTITUENTS FROM THE AERIAL PARTS OF LACTUCA TATARICA (L.) C.A. MEY. BY CHROMATOGRAPHY – MASS SPECTROMETRIC METHOD

Summary

This article is devoted to the investigation of the chemical components of the aerial parts (stems and leaves) of Lactuca tatarica. The phytochemical study of the aerial parts of the species L. tatarica (320 g) revealed the presence of coumarin derivatives in its composition. In the acetonic extraction of plant raw material the total extractive contents is investigated by the Liquid chromatography–mass spectrometric (LC-MS) method and coumarin derivatives have been identified. Totally 10 compounds were obtained, of which three were identified. It was found that, of these, other three compounds belong to coumarins, derivatives of polyphenols and phenylpropanoids. LC-MS experiments were realised by a Shimadzu 20 A HPLC system and identification of the substances were performed by Analyst 1.6 software. Gluconic acid, glucose, di-hydroxycoumarin glucoside, are amongst those which were identified. The importance of gluconic acid is that it is a chelator at alkaline pH. Moreover, it is used as a food additive in the food industry. The plant is widely used in traditional Chinese and Indian folk medicine in the treatment of certain diseases. Thus, we consider it important to study the chemical composition of a plant in order to detect biologically active components with broad pharmacological activity

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